6X7U

Crystal Structure of the Human Nudix Hydrolase Nudt16 in complex with FAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 32
Unit cell lengths	64.721, 64.721, 77.751
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.470 - 2.700
R-factor	0.1619
Rwork	0.159
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cou
RMSD bond length	0.006
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.500	2.870
High resolution limit [Å]	2.700	2.700
Rmerge	0.096
Number of reflections	9929	1602
<I/σ(I)>	11.4	1.65
Completeness [%]	99.5
Redundancy	4.3
CC(1/2)	0.996	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium chloride and 20% (w/v) PEG 3350