6X7O
LnmK in complex with 2-sulfonate-propionyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-02-17 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 61.110, 61.110, 312.261 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hzo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.999 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.088 | 1.352 |
| Number of reflections | 51831 | 5079 |
| <I/σ(I)> | 101.3 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.3 | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 3% PEG400, 67 mM Bis-Tris pH 6.5, 33 mM Bis-Tris pH 7.5, 28% PEG3350 |
