6X78
Vaccine-elicited mouse FP-targeting neutralizing antibody vFP48.03 in complex with HIV fusion peptide (residue 512-519)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.993, 92.733, 143.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.937 - 2.359 |
R-factor | 0.1802 |
Rwork | 0.178 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cdo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.400 |
High resolution limit [Å] | 2.359 | 6.400 | 2.360 |
Rmerge | 0.145 | 0.049 | 0.746 |
Rmeas | 0.157 | 0.054 | 0.817 |
Rpim | 0.061 | 0.021 | 0.327 |
Number of reflections | 45627 | 2479 | 2243 |
<I/σ(I)> | 5.5 | ||
Completeness [%] | 99.7 | 99.5 | 100 |
Redundancy | 6.6 | 6.1 | 6.1 |
CC(1/2) | 0.998 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris, pH 8.5, 25% w/v PEG3350 |