6X6O
Crystal structure of T4 protein Spackle as determined by native SAD phasing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 44.424, 79.822, 93.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.104 - 1.520 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.19070 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.033 | 1.471 |
| Number of reflections | 47113 | 1310 |
| <I/σ(I)> | 29.7 | 1.2 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 8.7 | 9 |
| CC(1/2) | 1.000 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.5 M ammonium sulfate, 0.05 M sodium acetate buffer, pH 4.5, and 4 % (v/v) glycerol |
