6X6F
The structure of Pf6r from the filamentous phage Pf6 of Pseudomonas aeruginosa PA01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-26 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.880, 160.310, 45.670 |
Unit cell angles | 90.00, 116.28, 90.00 |
Refinement procedure
Resolution | 40.950 - 1.735 |
R-factor | 0.1736 |
Rwork | 0.173 |
R-free | 0.19880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wnm |
RMSD bond length | 0.005 |
RMSD bond angle | 0.854 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.950 | 1.797 |
High resolution limit [Å] | 1.735 | 1.735 |
Rmerge | 0.062 | 0.527 |
Rmeas | 0.087 | 0.742 |
Rpim | 0.061 | 0.522 |
Number of reflections | 59270 | 5818 |
<I/σ(I)> | 12.3 | 2 |
Completeness [%] | 99.5 | 97.52 |
Redundancy | 3.8 | |
CC(1/2) | 0.997 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 295 | 20% Polyethylene glycol mono-methyl ether 5000, 200 mM Ammonium nitrate 10 mM HEPES, ph 7.5, 150 mM NaCl, 5% glycerol, 1 mM TCEP. 25% PEG 200 final cryoprotectant |