6X68
Cryo-EM structure of piggyBac transposase synaptic complex with hairpin DNA (SNHP)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | TUNGSTEN HAIRPIN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.66 |
| Number of Particle | 2623168 |
| Software | RELION,Rosetta,SerialEM |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
