6X60
ClpP2 from Chlamydia trachomatis with resolved handle loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-10-31 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.710, 147.043, 97.059 |
Unit cell angles | 90.00, 128.25, 90.00 |
Refinement procedure
Resolution | 28.160 - 2.810 |
R-factor | 0.2003 |
Rwork | 0.198 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jct |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18rc2_3794) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.096 | 0.712 |
Rpim | 0.049 | 0.374 |
Number of reflections | 34843 | 2318 |
<I/σ(I)> | 15.25 | 2.07 |
Completeness [%] | 99.8 | |
Redundancy | 3.8 | |
CC(1/2) | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | Tacsimate pH 7 |