6X5R
Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A2-Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 149.880, 149.880, 480.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.409 - 2.400 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2de0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.043 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.409 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.153 | 1.500 |
| Number of reflections | 124545 | 8849 |
| <I/σ(I)> | 18.4 | 1.4 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 10.4 | 11.7 |
| CC(1/2) | 0.999 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.0 M Lithium sulfate, 10 mM Nickel chloride, 100 mM Tris pH 8.5 |
