6X58
MPER-Fluc-Ec2 bound to 10E8v4 antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-09 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.976 |
Spacegroup name | P 32 |
Unit cell lengths | 99.050, 99.050, 167.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.020 - 3.260 |
R-factor | 0.2533 |
Rwork | 0.251 |
R-free | 0.29510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a43 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHASER |
Refinement software | REFMAC (1.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.180 | 38.180 | 3.460 |
High resolution limit [Å] | 3.260 | 9.780 | 3.260 |
Rmerge | 0.135 | 0.044 | 2.441 |
Rmeas | 0.141 | 0.046 | 2.558 |
Rpim | 0.043 | 0.014 | 0.763 |
Total number of observations | 306181 | 10949 | 51725 |
Number of reflections | 28368 | 1019 | 4601 |
<I/σ(I)> | 10.3 | 35.3 | 1 |
Completeness [%] | 98.6 | 95.1 | 98.4 |
Redundancy | 10.8 | 10.7 | 11.2 |
CC(1/2) | 0.999 | 0.999 | 0.765 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 34-39% PEG 300, 0.1 M NaCl, 0.1 M MES pH 6.2-7 |