6X2O
Crystal Structure of unliganded CRM1(E571K)-Ran-RanBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2016-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.293, 105.293, 306.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.391 - 2.551 |
| R-factor | 0.2224 |
| Rwork | 0.221 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hb2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 6.920 | 2.550 |
| Rmerge | 0.194 | 0.040 | 1.742 |
| Rmeas | 0.209 | 0.043 | 1.873 |
| Rpim | 0.078 | 0.017 | 0.682 |
| Total number of observations | 409472 | ||
| Number of reflections | 57265 | 3165 | 2802 |
| <I/σ(I)> | 3.9 | ||
| Completeness [%] | 99.9 | 99.3 | 99.9 |
| Redundancy | 7.2 | 6.5 | 7.5 |
| CC(1/2) | 0.993 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 17% (weight/vol) PEG3350, 100 mM Bis-Tris (pH 6.4), 200 mM ammonium nitrate, and 10 mM Spermine HCl |
