6X25
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 1-PHOSPHATASE INPP1 IN COMPLEX GADOLINIUM AFTER ADDITION OF INOSITOL 1,3,4-TRISPHOSPHATE AND LITHIUM AT 3.2 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 296 |
Detector technology | AREA DETECTOR |
Collection date | 1994-08-09 |
Detector | UCSD MARK II |
Wavelength(s) | 1.542 |
Spacegroup name | P 41 |
Unit cell lengths | 51.640, 51.640, 143.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.220 - 3.200 |
R-factor | 0.20161 |
Rwork | 0.199 |
R-free | 0.24551 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1inp |
RMSD bond length | 0.007 |
RMSD bond angle | 1.040 |
Data reduction software | UCSD-system |
Data scaling software | UCSD-system |
Phasing software | SQUASH |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.220 | 3.450 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 11374 | 950 |
<I/σ(I)> | 3.8 | |
Completeness [%] | 85.0 | |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 293 | Tetragonal shaped crystals amenable to diffraction studies were grown by the hanging drop vapor diffusion method on silanized glass covers slips at 20 C and grew with dimensions routinely exceeding 0.1 x 0.1 x 0.5mm. Gd3+, a competitive inhibitor of Mg2+, has a useful anomalous absorption edge at the CuK wavelength and therefore was used in the co-crystallization or soaking conditions. -Mg2+/+Li+/+Ins(1,3,4)P3/+Gd3+ (data=ip7r): Reservoir solution consisted of 10% PEG 8000, 80mM Bis-Tris, pH 5.25, 2mM Gd2(SO4)3, 1mM Ins(1,3,4)P3; 200mM Li2SO4 |