6X24

Structural basis of plant blue light photoreceptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 31
Unit cell lengths	208.362, 208.362, 81.607
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.050 - 3.250
R-factor	0.2732
Rwork	0.271
R-free	0.32850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u3c
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.050	3.360
High resolution limit [Å]	3.250	3.250
Rmeas	0.078	0.940
Number of reflections	61839	6050
<I/σ(I)>	14.8
Completeness [%]	99.3
Redundancy	1.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.5M Sodium Nitrate, 0.1M BIS-TRIS propane pH=7.0, 20% Glycerol