6X24
Structural basis of plant blue light photoreceptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 |
Unit cell lengths | 208.362, 208.362, 81.607 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.050 - 3.250 |
R-factor | 0.2732 |
Rwork | 0.271 |
R-free | 0.32850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u3c |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.050 | 3.360 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmeas | 0.078 | 0.940 |
Number of reflections | 61839 | 6050 |
<I/σ(I)> | 14.8 | |
Completeness [%] | 99.3 | |
Redundancy | 1.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.5M Sodium Nitrate, 0.1M BIS-TRIS propane pH=7.0, 20% Glycerol |