6X22
PDZ domain from choanoflagellate GIPC (mbGIPC) bound to GAIP peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977410 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.729, 35.028, 82.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.274 - 1.402 |
R-factor | 0.1885 |
Rwork | 0.187 |
R-free | 0.22130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v6b |
RMSD bond length | 0.006 |
RMSD bond angle | 0.867 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.274 | 1.590 | 1.490 |
High resolution limit [Å] | 1.402 | 1.490 | 1.402 |
Number of reflections | 17577 | 4779 | 4937 |
<I/σ(I)> | 22.08 | 3.23 | 1.68 |
Completeness [%] | 96.8 | 96 | 93.5 |
Redundancy | 6.79 | 6.9 | 6.52 |
CC(1/2) | 1.000 | 0.878 | 0.667 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 4% v/v Tacsimate, pH 4.0, 12% w/v PEG3350 |