6X1C
Tubulin-RB3_SLD-TTL in complex with compound 5j
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.345, 158.320, 181.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.140 - 2.900 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.21560 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 6br1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.527 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.209 | 1.310 |
Rpim | 0.060 | 0.372 |
Number of reflections | 69598 | 3470 |
<I/σ(I)> | 13.1 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.8 | 12.9 |
CC(1/2) | 0.992 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% PEG 4000, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |