6X0R
A Circular Permutant of the Tobacco Mosaic Virus (TMV) mutant Q101H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.1158 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 218.697, 176.213, 102.248 |
Unit cell angles | 90.00, 99.16, 90.00 |
Refinement procedure
Resolution | 48.970 - 3.000 |
R-factor | 0.2694 |
Rwork | 0.269 |
R-free | 0.28880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kml |
RMSD bond length | 0.002 |
RMSD bond angle | 0.451 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 136.520 | 2.860 |
High resolution limit [Å] | 2.810 | 2.810 |
Rmerge | 0.111 | |
Number of reflections | 91158 | 4016 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 98.0 | 88.2 |
Redundancy | 6.9 | 6 |
CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M SPG buffer (0.0125 M succinic acid, 0.0438 M sodium phosphate, 0.0437 M glycine), 12%-18% (v/v) PEG 1500, pH 5.0-5.5 |