6X08
Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 233.992, 233.992, 139.424 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 117.000 - 4.190 |
R-factor | 0.3131 |
Rwork | 0.311 |
R-free | 0.34650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ewe |
RMSD bond length | 0.006 |
RMSD bond angle | 0.949 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 117.000 | 4.350 |
High resolution limit [Å] | 4.190 | 4.190 |
Rpim | 0.056 | 0.665 |
Number of reflections | 17026 | 1665 |
<I/σ(I)> | 19.1 | 1.32 |
Completeness [%] | 100.0 | |
Redundancy | 37.9 | |
CC(1/2) | 0.982 | 0.642 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1M ammonium sulfate, di-sodium succinate pH 5.5, and the addition of 4% 1-propanol |