6X04

Nup133 (aa55-481) from S. cerevisiae bound by VHH-SAN5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	85.799, 204.761, 205.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.680 - 2.680
R-factor	0.2439
Rwork	0.243
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q9t
RMSD bond length	0.005
RMSD bond angle	0.647
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.680	2.800
High resolution limit [Å]	2.680	2.680
Rpim	0.035	0.684
Number of reflections	100374	9937
<I/σ(I)>	29.1	1.49
Completeness [%]	98.7	99.1
Redundancy	8.6	8.3
CC(1/2)	0.999	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% PEG MME 2,000, 0.1M potassium chloride, 0.1M Tris/HCl pH 8