6X03
Nup84-Nup133 (aa521-1157) from S. cerevisiae bound by VHH-SAN8 and VHH-SAN9
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.252, 295.205, 295.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 147.800 - 7.300 |
R-factor | 0.3365 |
Rwork | 0.335 |
R-free | 0.35340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | unpublished |
RMSD bond length | 0.003 |
RMSD bond angle | 0.809 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 147.800 | 7.710 |
High resolution limit [Å] | 7.300 | 7.320 |
Rpim | 0.030 | 0.597 |
Number of reflections | 9375 | 1263 |
<I/σ(I)> | 41.9 | 1.25 |
Completeness [%] | 99.7 | |
Redundancy | 11.9 | |
CC(1/2) | 0.988 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate |