6X02

Nup84-Nup133 (aa521-1157) from S. cerevisiae bound by VHH-SAN8

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	72.257, 287.650, 297.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.380 - 6.380
R-factor	0.3149
Rwork	0.314
R-free	0.32420
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.840
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	103.400	8.700	6.630
High resolution limit [Å]	6.400	7.800	6.380
Rpim	0.022	0.305	0.813
Number of reflections	13539	2067	1322
<I/σ(I)>	55.3	2.1	1
Completeness [%]	99.8
Redundancy	24.1
CC(1/2)	1.000	0.891	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate
1	VAPOR DIFFUSION, SITTING DROP		291	6% PEG 8,000, 30% ethylene glycol, 0.1M imidazole titrated with MES pH 6.5, 15mM sodium nitrate, 15mM sodium phosphate dibasic, 15mM ammonium sulfate