6X01
Crystal structure of the GltPh V216C-A391C mutant cross-linked in outward-facing state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 61 |
Unit cell lengths | 113.985, 113.985, 321.705 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.840 - 3.650 |
R-factor | 0.2733 |
Rwork | 0.272 |
R-free | 0.30860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nwx |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.790 | 3.900 |
High resolution limit [Å] | 3.650 | 3.650 |
Rmerge | 0.128 | 3.559 |
Number of reflections | 26118 | 4731 |
<I/σ(I)> | 14 | 1.2 |
Completeness [%] | 100.0 | |
Redundancy | 21.6 | |
CC(1/2) | 0.999 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 15/23% PEG 1000, 100mM Lithium sulfate, 50mM citric acid, 50mM Disodium phosphate |