6WZZ
GID4 in complex with VGLWKS peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.781, 40.648, 56.319 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 36.403 - 1.600 |
| Rwork | 0.186 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ccr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.670 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.650 | 40.650 | 1.620 |
| High resolution limit [Å] | 1.600 | 8.750 | 1.600 |
| Rmerge | 0.047 | 0.030 | 0.588 |
| Rmeas | 0.056 | 0.036 | 0.787 |
| Rpim | 0.031 | 0.020 | 0.518 |
| Total number of observations | 65453 | 461 | 1291 |
| Number of reflections | 21226 | 153 | 746 |
| <I/σ(I)> | 13.8 | 37.5 | 1.3 |
| Completeness [%] | 96.3 | 98.5 | 68.5 |
| Redundancy | 3.1 | 3 | 1.7 |
| CC(1/2) | 0.998 | 0.998 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.03 M Citric acid, pH 7.6 and 20% (w/v) PEG 3350 |
