6WZO

Structure of SARS-CoV-2 Nucleocapsid dimerization domain, P1 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	43.718, 50.061, 69.337
Unit cell angles	106.50, 90.09, 97.14

Refinement procedure

Resolution	66.430 - 1.420
R-factor	0.1581
Rwork	0.157
R-free	0.17320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gib
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.430	1.440
High resolution limit [Å]	1.420	1.420
Rmerge		0.310
Rmeas	0.046	0.363
Number of reflections	96446	3939
<I/σ(I)>	16.8	3
Completeness [%]	91.1
Redundancy	3.7
CC(1/2)	0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Sodium acetate pH 4.5, 50 mM Sodium/potassium tartrate, and 34% polyethylene glycol PEG 3350