6WZB
Crystal structure of the GltPh V216C-G388C mutant cross-linked with divalent mercury
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 112.111, 204.573, 207.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.311 - 3.450 |
R-factor | 0.2343 |
Rwork | 0.233 |
R-free | 0.26770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kbc |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.311 | 3.640 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.155 | 1.518 |
Number of reflections | 31780 | 4587 |
<I/σ(I)> | 10.3 | 2.2 |
Completeness [%] | 100.0 | |
Redundancy | 13.8 | |
CC(1/2) | 0.997 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 30/35% PEG 400, 0.2M magnesium chloride, 0.1M potassium chloride, 0.025M sodium citrate |