6WZ2
Co-bound structure of an engineered protein trimer, TriCyt3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.239800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.252, 78.105, 49.562 |
Unit cell angles | 90.00, 106.64, 90.00 |
Refinement procedure
Resolution | 35.048 - 2.001 |
R-factor | 0.1783 |
Rwork | 0.175 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bc5 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.876 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.048 | 2.073 |
High resolution limit [Å] | 2.001 | 2.001 |
Rmerge | 0.060 | 0.425 |
Rmeas | 0.065 | 0.467 |
Rpim | 0.025 | 0.189 |
Number of reflections | 23044 | 1947 |
<I/σ(I)> | 20.34 | 3.64 |
Completeness [%] | 96.6 | |
Redundancy | 6.7 | |
CC(1/2) | 0.999 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25% PEG1500, 0.2 M MgCl2, 0.1 M HEPES (pH 7.5) |