6WZ0
Fe-bound structure of an engineered metal-dependent protein trimer, TriCyt2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.953680 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.206, 79.260, 49.916 |
Unit cell angles | 90.00, 106.89, 90.00 |
Refinement procedure
Resolution | 47.782 - 1.700 |
R-factor | 0.1834 |
Rwork | 0.182 |
R-free | 0.21180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bc5 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.841 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.782 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.433 | 0.499 |
Rmeas | 0.470 | 0.540 |
Rpim | 0.180 | 0.206 |
Number of reflections | 76338 | 3931 |
<I/σ(I)> | 2.94 | 2.11 |
Completeness [%] | 98.7 | 99.7 |
Redundancy | 6.3 | 6 |
CC(1/2) | 0.910 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 25% PEG400, 0.2 M (NH4)2SO4, 0.1 M TRIS (pH 8.5) |