6WYR
Crystal structure of anti-Muscle Specific Kinase (MuSK) Fab, MuSK1A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-03-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.870, 71.720, 106.180 |
Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
Resolution | 49.040 - 1.800 |
R-factor | 0.1812 |
Rwork | 0.180 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dw2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.040 | 49.040 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.073 | 0.021 | 1.089 |
Rmeas | 0.086 | 0.025 | 1.304 |
Total number of observations | 306753 | ||
Number of reflections | 88524 | 1048 | 6403 |
<I/σ(I)> | 9.68 | 40.19 | 0.87 |
Completeness [%] | 99.5 | 98.1 | 98.5 |
Redundancy | 3.465 | 3.442 | 3.238 |
CC(1/2) | 0.998 | 0.999 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | 20% PEG6000, 0.1 M bicine, pH 8.5 |