6WYE

Crystal structure of Neisseria gonorrhoeae serine acetyltransferase (CysE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 2 1
Unit cell lengths	77.897, 93.867, 102.019
Unit cell angles	90.00, 91.29, 90.00

Refinement procedure

Resolution	43.110 - 2.010
R-factor	0.1849
Rwork	0.183
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gvd
RMSD bond length	0.008
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.930	2.040
High resolution limit [Å]	2.010	2.010
Rpim	0.023
Number of reflections	97704	4841
<I/σ(I)>	17.2	3.5
Completeness [%]	100.0	100
Redundancy	7.1
CC(1/2)	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	291	28% (v/v) Tacsimate