6WYC
Crystal Structure of Chlamydia trachomatis Glyceraldehyde 3-phosphate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.603, 104.053, 86.473 |
| Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
| Resolution | 66.260 - 1.500 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.18850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qx6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.260 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.076 | 0.626 |
| Rmeas | 0.089 | 0.742 |
| Rpim | 0.045 | 0.388 |
| Number of reflections | 173217 | 8596 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 93.2 | 93 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.997 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 25% PEG 2000 MME, 0.1 M Hepes, pH 7.5; prior to crystallization the protein (at 30 mg/ml) was incubated with 1 mM NAD |
