6WY6

Crystal structure of S. cerevisiae Atg8 in complex with Ede1 (1220-1247)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 43
Unit cell lengths	49.642, 49.642, 123.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.057 - 1.773
R-factor	0.1771
Rwork	0.176
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2znp
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (ccp4)
Refinement software	REFMAC (ccp4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.060	1.836
High resolution limit [Å]	1.773	1.773
Rmerge	0.054	0.409
Rmeas	0.068	0.586
Rpim	0.032	0.297
Number of reflections	28870	2848
<I/σ(I)>	29.36
Completeness [%]	100.0	99.65
Redundancy	8.8	7.6
CC(1/2)	0.996	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277.15	1.1 M ammonium sulfate, 0.1 M sodium acetate, pH 4.8, 0.01 M sodium iodide