6WXX
crystal structure of cA4-activated Card1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.064, 121.216, 103.708 |
Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
Resolution | 28.880 - 3.000 |
R-factor | 0.2053 |
Rwork | 0.204 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | apo Cad1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.803 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.143 | 1.215 |
Rmeas | 0.165 | 1.421 |
Rpim | 0.082 | 0.726 |
Number of reflections | 38486 | 38486 |
<I/σ(I)> | 8.06 | 1.06 |
Completeness [%] | 98.7 | |
Redundancy | 3.9 | |
CC(1/2) | 0.994 | 0.457 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M citric acid, 10% PEG6000 (v/v), final pH 5.0 |