6WXW
crystal structure of apo Card1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.152, 124.709, 42.643 |
Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
Resolution | 86.450 - 2.320 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24430 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.826 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.450 | 2.410 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.078 | 0.760 |
Rmeas | 0.090 | 0.886 |
Rpim | 0.045 | 0.449 |
Number of reflections | 38510 | 3739 |
<I/σ(I)> | 12.93 | 1.6 |
Completeness [%] | 98.8 | |
Redundancy | 3.9 | |
CC(1/2) | 0.997 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M K2HPO4, and 20% PEG3350 (v/v) |