6WXW

crystal structure of apo Card1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	174.152, 124.709, 42.643
Unit cell angles	90.00, 96.88, 90.00

Refinement procedure

Resolution	86.450 - 2.320
R-factor	0.207
Rwork	0.205
R-free	0.24430
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.826
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.450	2.410
High resolution limit [Å]	2.320	2.320
Rmerge	0.078	0.760
Rmeas	0.090	0.886
Rpim	0.045	0.449
Number of reflections	38510	3739
<I/σ(I)>	12.93	1.6
Completeness [%]	98.8
Redundancy	3.9
CC(1/2)	0.997	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M K2HPO4, and 20% PEG3350 (v/v)