6WXK
PHF23 PHD Domain Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-26 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.916, 66.916, 151.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.230 - 2.900 |
| Rwork | 0.241 |
| R-free | 0.29380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o70 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.060 | 0.320 |
| Number of reflections | 8025 | 763 |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 97.8 | 97.1 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.65 | 291 | 0.1 M Tris pH 8.65 and 25% tert-butanol |
