6WX6
Cryo-EM Structure of Human Apoferritin Light Chain Vitrified Using Back-it-up
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2 |
| Number of Particle | 673794 |
| Software | cryoSPARC,Coot,PHENIX,EPU,UCSF Chimera |
| Symmetry | O |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
