6WX1

Antigen Binding Fragment of OKT9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	94.660, 113.960, 40.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.820 - 2.000
R-factor	0.22
Rwork	0.216
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	OKT9_Fab_P22121_refine_13.pdb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.820	48.820	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.057	0.027	0.600
Rmeas	0.064	0.031	0.669
Total number of observations	154169
Number of reflections	30479	408	2223
<I/σ(I)>	15.66	38.66	3.12
Completeness [%]	99.3	98.3	98.3
Redundancy	5.058	4.355	5.093
CC(1/2)	0.999	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM TRIS, 190mM Magnesium chloride, 26% (w/v) PEG 8,000