6WVI

VKOR-like from Takifugu rubripes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	153.639, 49.064, 84.326
Unit cell angles	90.00, 112.73, 90.00

Refinement procedure

Resolution	35.430 - 2.400
R-factor	0.1986
Rwork	0.197
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.397	6.510	2.400
Rmerge	0.118	0.040	1.392
Rmeas	0.136	0.046	1.616
Rpim	0.067	0.023	0.804
Number of reflections	22666	1178	1154
<I/σ(I)>	8.6
Completeness [%]	99.0	96.6	99.5
Redundancy	4	3.9	3.8
CC(1/2)		0.997	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5	295	42% PEG 400, 50 mM ammonium formate, 0.1 M HEPES pH 7.5