6WV2
Crystal Structure of Streptococcal Bacteriophage Hyaluronidase: Presence of a Prokaryotic Collagen and Elucidation of Catalytic Mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.451, 86.789, 168.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.609 - 2.210 |
Rwork | 0.213 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c3f |
RMSD bond length | 0.006 |
RMSD bond angle | 0.997 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.087 | 0.483 |
Number of reflections | 50393 | 4986 |
<I/σ(I)> | 9.4 | 5.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 10% (V/V) 2-PROPANOL AND 20% (W/V) PEG 4000 IN 0.1 M HEPES, PH 7.5 |