6WUK
Crystal structure of mouse DXO in complex with CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.862, 87.665, 54.023 |
Unit cell angles | 90.00, 112.24, 90.00 |
Refinement procedure
Resolution | 50.005 - 1.599 |
R-factor | 0.1856 |
Rwork | 0.185 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j7l |
RMSD bond length | 0.012 |
RMSD bond angle | 1.168 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.005 | 50.005 | 1.640 |
High resolution limit [Å] | 1.599 | 7.150 | 1.600 |
Rmerge | 0.047 | 0.023 | 0.757 |
Rmeas | 0.055 | 0.026 | 0.884 |
Number of reflections | 56513 | 663 | 4081 |
<I/σ(I)> | 13.73 | 40.6 | 1.56 |
Completeness [%] | 99.4 | 98.8 | 97.4 |
Redundancy | 3.771 | 3.748 | 3.675 |
CC(1/2) | 0.999 | 0.999 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23% (w/v) PEG3350 |