6WU7

Human Calcium and Integrin Binding Protein 3 E150Q K151H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.46
Spacegroup name	P 31 2 1
Unit cell lengths	84.386, 84.386, 125.821
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.080 - 1.840
R-factor	0.163
Rwork	0.162
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wu5
RMSD bond length	0.010
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.080	1.902
High resolution limit [Å]	1.837	1.837
Number of reflections	44453	3797
<I/σ(I)>	42.15	7.12
Completeness [%]	96.9	84.12
Redundancy	18
CC(1/2)	0.998	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% PEG 8000 0.2M Calcium Acetate