6WTV

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 258259

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	93.800, 98.654, 103.094
Unit cell angles	114.15, 105.00, 89.69

Refinement procedure

Resolution	45.006 - 3.050
R-factor	0.2373
Rwork	0.235
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.166	49.166	3.230
High resolution limit [Å]	3.050	9.000	3.050
Rmerge	0.178	0.058	0.516
Rmeas	0.226	0.073	0.655
Total number of observations	151296
Number of reflections	60526	2318	9524
<I/σ(I)>	5.16	14.87	1.97
Completeness [%]	97.4	95.9	94.9
Redundancy	2.5	2.596	2.469
CC(1/2)	0.971	0.993	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.4 M ammonium nitrate, 18% (w/v) PEG 3,350