6WTL
Structure of Human pir-miRNA-19b-2 Apical Loop and One-base-pair Fused to the YdaO Riboswitch Scaffold
Replaces: 6N5LExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 115.340, 115.340, 115.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.491 - 2.850 |
R-factor | 0.1898 |
Rwork | 0.187 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qk8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.766 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.491 | 2.920 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmeas | 0.061 | 1.620 |
Rpim | 0.014 | 0.358 |
Number of reflections | 21107 | 1386 |
<I/σ(I)> | 32.8 | 2 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 19.8 | 17.9 |
CC(1/2) | 0.999 | 0.816 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 297 | 1.7 M (NH4)2SO4, 0.2 M LI2SO4, AND 0.1 M HEPES PH 7.1 |