6WT5

Structure of a bacterial STING receptor from Capnocytophaga granulosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	123.709, 123.709, 108.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 2.800
R-factor	0.237
Rwork	0.233
R-free	0.28130
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	2.950
High resolution limit [Å]	2.800	2.800
Rpim	0.032	0.702
Number of reflections	21303	3034
<I/σ(I)>	15.4
Completeness [%]	100.0
Redundancy	27
CC(1/2)	0.999	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1 M HEPES-NaOH pH 7.5, 20% PEG-10000