6WT4
Structure of a bacterial STING receptor from Flavobacteriaceae sp. in complex with 3',3'-cGAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-08-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 4 |
Unit cell lengths | 121.848, 121.848, 48.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.080 - 1.780 |
R-factor | 0.2004 |
Rwork | 0.199 |
R-free | 0.23030 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.632 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.790 | 1.820 |
High resolution limit [Å] | 1.780 | 1.780 |
Rpim | 0.019 | 0.531 |
Number of reflections | 34093 | 1916 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 99.9 | |
Redundancy | 13.8 | |
CC(1/2) | 0.999 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 M ammonium sulfate, 0.2 M sodium acetate pH 4.5 |