6WRY
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 1-PHOSPHATASE INPP1 IN COMPLEX GADOLINIUM AFTER ADDITION OF INOSITOL 1,3,4-TRISPHOSPHATE AT 2.5 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 296 |
Detector technology | AREA DETECTOR |
Collection date | 1994-07-22 |
Detector | UCSD MARK II |
Wavelength(s) | 1.542 |
Spacegroup name | P 41 |
Unit cell lengths | 51.640, 51.640, 143.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.950 - 2.800 |
R-factor | 0.20742 |
Rwork | 0.205 |
R-free | 0.25027 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1inp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.041 |
Data reduction software | UCSD-system |
Data scaling software | UCSD-system |
Phasing software | SQUASH |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.950 | 3.020 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 15787 | 1210 |
<I/σ(I)> | 4.2 | |
Completeness [%] | 79.0 | 70 |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 293 | -Mg2+/-Li+/+Ins(1,3,4)P3/+Gd3+ (data=ip1r): Reservoir solution consisted of 10% PEG 8000, 80mM Bis-Tris, pH 5.25, 2mM Gd2(SO4)3, 1mM Ins(1,3,4)P3 |