6WQY
GH5-4 broad specificity endoglucanase from Bacteroides salanitronis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.7749 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.480, 92.310, 95.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.240 - 1.050 |
R-factor | 0.1389 |
Rwork | 0.139 |
R-free | 0.14530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhe |
RMSD bond length | 0.005 |
RMSD bond angle | 0.882 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.240 | 1.088 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.051 | 0.832 |
Rmeas | 0.053 | 0.911 |
Rpim | 0.015 | 0.358 |
Number of reflections | 165260 | 15327 |
<I/σ(I)> | 22.62 | 1.68 |
Completeness [%] | 99.2 | 93 |
Redundancy | 12.1 | 6.1 |
CC(1/2) | 1.000 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 200 nL of a solution containing 59 mg/mL protein, 50 nM NaCl, 5 mM MOPS buffer pH 7 was mixed with 130 nL reservoir solution containing 16% PEG3350, 0.2 M MgCl2, and 0.1 M sodium acetate buffer at pH 5. Vapor diffusion in MRC plates. Crystals appeared within a few days, and were cryo-protected using a reservoir solution supplemented to 35% PEG3350. |