6WQW
Thermobacillus composti GH10 xylanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.4586 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 81.264, 81.264, 92.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.819 - 2.102 |
R-factor | 0.2364 |
Rwork | 0.235 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n82 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.820 | 2.180 |
High resolution limit [Å] | 2.102 | 2.102 |
Number of reflections | 18595 | 1809 |
<I/σ(I)> | 18.26 | 2.23 |
Completeness [%] | 98.4 | 99.78 |
Redundancy | 22.7 | 19.4 |
CC(1/2) | 1.000 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, 70% (v/v) 2-Methyl-2,4-pentanediol (MPD) |