6WQV

GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978560
Spacegroup name	P 1
Unit cell lengths	49.610, 83.170, 85.210
Unit cell angles	93.98, 93.36, 89.27

Refinement procedure

Resolution	36.890 - 1.450
R-factor	0.1512
Rwork	0.151
R-free	0.16900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wqp
RMSD bond length	0.005
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.890	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.046	0.433
Rmeas	0.053	0.509
Rpim	0.026	0.262
Number of reflections	224235	16603
<I/σ(I)>	17	2.79
Completeness [%]	93.2	68.84
Redundancy	4.2
CC(1/2)	0.999	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% PEG3350, BisTris Propane pH 7.0 and 200 mM Sodium Nitrate, 1 mM cellotriose, cryoprotected in above supplemented with 15% ethylene glycol.