6WQC

Crystal structure of VipF from Legionella hackeliae in complex with CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 2
Unit cell lengths	172.062, 36.659, 59.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.340
R-factor	0.1849
Rwork	0.182
R-free	0.23320
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	2.104
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.095	0.689
Rpim	0.028	0.248
Number of reflections	15984	660
<I/σ(I)>	24.76	2
Completeness [%]	95.0
Redundancy	11.6
CC(1/2)	0.993	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1 M ammonium citrate pH 7.0, 0.1 M Bis-Tris pH 7, 0.2 M ammonium acetate, Cryo: paratone