6WPN

Crystal structure of a putative oligosaccharide periplasmic-binding protein from Synechococcus sp. MITs9220

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	59.947, 69.525, 83.721
Unit cell angles	90.00, 94.70, 90.00

Refinement procedure

Resolution	41.720 - 2.290
R-factor	0.2042
Rwork	0.201
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wpm
RMSD bond length	0.004
RMSD bond angle	0.682
Data reduction software	Aimless
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.790	46.790	2.380
High resolution limit [Å]	2.290	8.900	2.380
Rmerge	0.072
Rmeas	0.083
Rpim	0.042
Number of reflections	30536	549	2892
<I/σ(I)>	10.5
Completeness [%]	99.5	98	96.5
Redundancy	3.8	3.6	3.6
CC(1/2)	0.999	0.999	0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Ammonium sulphate (2 M), Tris (0.1 M, pH 8.5)