6WPM
Crystal structure of a putative oligosaccharide periplasmic-binding protein from Synechococcus sp. MITs9220 in complex with zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-03-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 144.778, 144.778, 53.716 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.780 - 2.880 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25390 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Data reduction software | pointless |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.420 | 2.980 |
| High resolution limit [Å] | 2.230 | 2.880 |
| Rmerge | 0.072 | 0.027 |
| Rmeas | 0.038 | |
| Rpim | 0.027 | |
| Number of reflections | 13405 | 1253 |
| <I/σ(I)> | 0.0694 | 30 |
| Completeness [%] | 99.4 | 94.6 |
| Redundancy | 3.8 | 2 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | Ammonium sulphate (2 M), PEG 3350 (20% w/v) |
