6WPJ

Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and d4T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	167.588, 170.061, 102.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.560 - 2.730
R-factor	0.2059
Rwork	0.203
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6or7
RMSD bond length	0.007
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.730	2.730
Number of reflections	38882	5942
<I/σ(I)>	12.46	1.56
Completeness [%]	99.1	94.8
Redundancy	7.2	6.69
CC(1/2)	0.996	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0